The eight-time Olympic gold medalist is likely to be the fastest player in the game.
Any gamer who pre-orders PES, which is released on September 14, will be given access to use the Jamaican as a player.
Bolt said: “I love football and have played PES for as long as I can remember – it’s the best football game there is – and it’s a great honour to be a part of it and its success.
“When the opportunity arose to be a player in PES 2018, it was too good to be true.
“Having my face and movements scanned for use in the game was a fascinating process and I hope those who pre-order the game make full use of my pace and skill!”
Bolt, who is retiring from athletics after the World Championships in London this summer, will also have a chance to take on Lionel Messi – albeit virtually.
Last week, Bolt challenged the Barcelona ace to a race.
He said: “I always like to try new things and Messi is one of the greatest footballers to ever grace the face of this earth.
“It would be an honour [to race him] because I have never met Messi.”